Batman is a DC Comics superhero who first appeared in Detective Comics #27 in May 1939. His true identity is Bruce Wayne, billionaire industrialist, playboy, and philanthropist. Witnessing the murder of his parents as a child led him to train himself to the peak of physical and intellectual perfection, don a costume, and fight crime. Unlike many other superheroes, he does not possess superhuman powers or abilities; he makes use of intellect, detective skills, technology, and physical prowess in his war on crime.
- "Inside Plucky Duck" - The segment "Bat's All Folks" features Plucky as Batduck and Hamton as Decoy, parodies of Batman and Robin.
- "The Return of Batduck" - The episode's plot centers on Plucky trying to convince Tim Burton to give him the starring role in Batman Returns.
- "A Midsummer Night's Dream" - Dot translates Yakko's last line as "And the Boy Wonder will save us." Robin then arrives in the Batmobile and drives the Warners out.
- "Dance of Doom" - As Joe Leahy announces Cave Guy's appearance, he remarks that only one hero can stop him, and the camera to show a silhouetted Batman stopping a thug. "That hero is... on another network," Joe remarks. This is a reference to the fact that, at the time Freakazoid! premiered, Batman: The Animated Series was still on Fox Kids.
- "Fatman and Boy Blubber" - Fatman's theme song is sung to the tune of the theme from the 1960s Batman TV series.
- "The Renaissance" - Leonardo da Vinci stars in a sketch featuring him as Renaissance Man, with Loud Kiddington dressed as Robin. The theme from the 1960s Batman series is also parodied.
- "The Legion of Super Writers" - During the "Super Writers" sketch, Big Fat Baby wears a Batman mask.
- "Americana" - When calling Barry Ding, Froggo is shown in his bedroom, which is decorated with Batman merchandise.
- Looney Tunes: Back in Action - While being chased by DJ Drake, Daffy dashes onto the set of a Batman film and turns on the Batmobile, causing to drive off and crash into the WB Water Tower.
- Edward Asner played Roland Daggett in Batman: The Animated Series (1992-1995)
- Laraine Newman played Baby Doll in an episode of The New Batman/Superman Adventures (1997)
- Cree Summer played Maxine "Max" Gibson in Batman Beyond (1999-2001)
- David Warner played Ra's Al Ghul in Batman: The Animated Series (1992-2000)
- Frank Welker played Isis the Cat, Ace the Bathound, and other animal and speaking roles in Batman: the Animated Series (1992-1995) and Batman Beyond (1999-2001)
- Adam West portrayed Batman in the 1960s TV series.